The current standard methods delimit the M-spike component based on the projected gel image on screen (current standard method, CSM). Triple-target stimuli-responsive anti-COVID-19 face mask with physiological virus-inactivating agents (2021)Context: Serum monoclonal immunoglobulin (M-spike) in multiple myeloma is measured by gel electrophoresis (SPE) followed by densitometric scanning of the gel. Pandalis Is Highly Effective Against Delta Variant of SARS-CoV-2 in Vitro (2021)ġ1. A Novel Therapeutic Peptide Blocks SARS-CoV-2 Spike Protein Binding with Host Cell ACE2 Receptor (2021)ġ0. Discovery and evaluation of entry inhibitors for SARS-CoV-2 and its emerging variants (2021)ĩ. GB-2 blocking the interaction between ACE2 and wild type and mutation of spike protein of SARS-CoV-2 (2021)Ĩ. Identification of natural compounds as SARS-CoV-2 entry inhibitors by molecular docking-based virtual screening with bio-layer interferometry (2021)ħ. Corilagin prevents SARS-CoV-2 infection by targeting RBD-ACE2 binding (2021)Ħ.
Discovery of a AHR pelargonidin agonist that counter-regulates Ace2 expression and attenuates ACE2-SARS-CoV-2 interaction (2021)ĥ. Clomipramine suppresses ACE2-mediated SARS-CoV-2 entry (2021)Ĥ. ACE-2-interacting Domain of SARS-CoV-2 (AIDS) Peptide Suppresses Inflammation to Reduce Fever and Protect Lungs and Heart in Mice: Implications for COVID-19 Therapy (2021)ģ. Hijacking SARS-CoV-2/ACE2 Receptor Interaction by Natural and Semi-synthetic Steroidal Agents Acting on Functional Pockets on the Receptor Binding Domain (2020)Ģ. Finally, the plate is treated with Anti-mouse-Fc-HRP followed by addition of an HRP substrate to produce chemiluminescence, which then can be measured using a chemiluminescence reader. Next, SARS-CoV-2 Spike S1 is incubated with ACE2 on the plate. First, ACE2 protein is attached to a nickel-coated 96-well plate. Only a few simple steps on a microtiter plate are required for the assay. The ACE2:SARS-CoV-2 Spike S1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is the high sensitivity of detection of Fc-tagged Spike protein by HRP-labeled Anti-mouse-Fc. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection. The Spike protein receptor binding domain (RBD) recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. In this instance, we suggest using the SARS-CoV-2 Spike:ACE2 Inhibitor Screening Assay Kit, BPS Bioscience #79945.Ĭoronavirus disease 2019 (COVID-19) increases the risk of developing Acute Respiratory Distress Syndrome (ARDS), which is often fatal at the late stages of the infection when the SAR-CoV-2 virus causes significant damage to the lungs.
If your sample includes human serum, it may interfere with the assay and create a false positive signal. This kit is based on binding to the mouse Fc region.
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